RBPJ Antibody (N-term)

• Catalog Number: orb1934060
• Category: Antibodies
• Description: Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB36620
• Tested applications: IF, IHC-P, WB
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Conjugation: Unconjugated
• MW: 55637 Da
• Target: This RBPJ antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1-29 amino acids from the N-terminal region of human RBPJ.
• UniProt ID: Q06330
• NCBI: NP_056958.3, NP_005340.2, NP_976029.1, NP_976028.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Recombining binding protein suppressor of hairless
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

RBPJ Antibody (N-term) western blot analysis in Ramos cell line lysates (35 ug/lane). This demonstrates the RBPJ antibody detected the RBPJ protein (arrow).

Anti-RBPJ Antibody (N-term) at 1:2000 dilution + human pancreas lysates. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 56 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.

RBPJ Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RBPJ Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.

Fluorescent confocal image of A2058 cell stained with RBPJ Antibody (N-term). A2058 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with RBPJ primary antibody (1:25, 1 h at 37°C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37°C). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7 units/ml, 1 h at 37°C). Nuclei were counterstained with DAPI (blue) (10 µg/ml, 10 min). RBPJ immunoreactivity is localized to nucleus significantly.