RB1 Antibody

• Catalog Number: orb1262629
• Category: Antibodies
• Description: RB1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human
• Isotype: Rabbit Ig
• Immunogen: This RB1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 858-886 amino acids from the C-terminal region of human RB1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 106 kDa
• Target: RB1
• UniProt ID: P06400
• NCBI: P06400
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: Retinoblastoma-associated protein, p105-Rb, pRb, R
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For IHC-P starting dilution is: 1:50~100For FACS starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western Blot at 1:8000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

Western blot analysis of RB1 Antibody in 293 cell line lysates (35 ug/lane) (2ug/ml)

Formalin-fixed and paraffin-embedded human testis tissue reacted with RB1 Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.

Flow cytometric analysis of 293 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.