Rb (phospho-Ser249) antibody

• Catalog Number: orb769875
• Category: Antibodies
• Description: Rabbit polyclonal antibody to Rb (phospho-Ser249)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: The antiserum was produced against synthesized peptide derived from human Retinoblastoma around the phosphorylation site of Ser249. AA range:221-270
• Concentration: 1 mg/ml
• Conjugation: Unconjugated
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.
• Alternative names: Anti-RB1 antibody, anti-Retinoblastoma-associated
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of HeLa cells, using Retinoblastoma (Phospho-Ser249) Antibody. The picture on the right is blocked with the phospho peptide.

Immunohistochemical analysis of paraffin-embedded human cervical carcinoma. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).