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Catalog Number | orb779827 |
---|---|
Category | Assays and Kits |
Description | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Peptide YY(PYY) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Peptide YY(PYY). |
Tested applications | ELISA |
Reactivity | Rat |
Range | 15.63-1000 pg/mL |
Concentration | 1000 pg/mL |
UniProt ID | P10631 |
Sensitivity | 4.83 pg/mL |
Protein Length | 2h |
Alternative names | PYY1 ELISA Kit, Peptide tyrosine tyrosine ELISA Ki Read more... |
Note | For research use only |
Application notes | standard: 1000 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat PYY protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat PYY. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat PYY in the samples is then determined by comparing the OD of the samples to the standard curve |
Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids |
Expiration Date | Please enquire. |
Rat | |
7.8 pg/mL-500 pg/mL | |
1.95 pg/mL |