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Catalog Number | orb343820 |
---|---|
Category | Proteins |
Description | Rat IgG1 isotype control Biotin Antibody |
Tested applications | DOT, ELISA, FC, WB |
Isotype | IgG1 |
Concentration | 1 mg/mL |
Dilution range | ELISA: User Optimized, FC: User Optimized, WB: User Optimized |
Form/Appearance | Lyophilized |
Purity | RAT IgG1 isotype control has been prepared from concentrated cell culture supernatant by immunoaffinity chromatography using protein G. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rat IgG, and anti-Rat serum. Isotyping assay resulted non-reactive with antisera to Rat IgG2a, IgG2b, IgG3, IgM, and IgA. Biotin Conjugated Rat IgG1 confirmed by Dot Blot. |
Conjugation | Biotin |
Source | Rat |
Biological Origin | Rat |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Rat Isotype Control, Rat IgG1 Biotin isotype, RAT Read more... |
Note | For research use only |
Application notes | RAT IgG1 isotype control has been tested in dot blot and can be utilized as a control or standard reagent in Western Blotting, Flow Cytometry, and ELISA experiments where determination of sample isotype is important. Specific conditions should be optimized by user. |
Expiration Date | 12 months from date of receipt. |
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