RAT IgG (H&L) antibody (Peroxidase)

• Catalog Number: orb347881
• Category: Antibodies
• Description: RAT IgG (H&L) antibody (Peroxidase)
• Species/Host: Sheep
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Rat
• Isotype: IgG
• Immunogen: Rat IgG whole molecule
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: 1:60,000 - 1:100,000, IHC: 1:500 - 1:2,500, WB: 1:1,000 - 1:10,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum, Rat IgG and Rat Serum.
• Conjugation: HRP
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
• Alternative names: Sheep Anti-Rat IgG peroxidase Conjugated Antibody,
• Note: For research use only
• Application notes: Anti-Rat IgG Peroxidase Conjugated Antibody has been tested by ELISA and is assayed against 1.0 µg of Rat IgG in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:5,000 to 1:25,000 of the reconstitution concentration is suggested for this product.
• Expiration Date: 12 months from date of receipt.

Western Blot of Anti-Rat IgG (H&L) (SHEEP) Antibody (p/n orb347879). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (SHEEP) Antibody (p/n orb347879) 1:1000 for 60 min at RT. Secondary Antibody: Anti-Sheep IgG (DONKEY) Peroxidase Conjugated Antibody (p/n orb347953) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.