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Rat IgG (H&L) Antibody Biotin Conjugated

Catalog Number: orb347833

DispatchUsually dispatched within 5-10 working days
$ 300.00
Catalog Numberorb347833
CategoryAntibodies
DescriptionRat IgG (H&L) antibody (Biotin)
Species/HostGoat
ClonalityPolyclonal
Tested applicationsELISA, IHC, WB
ReactivityRat
IsotypeIgG
ImmunogenRat IgG whole molecule
Concentration2.0 mg/mL
Dilution rangeELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000
Form/AppearanceLyophilized
PurityAnti-RAT IgG Biotin Conjugated antibody was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Goat Serum, Rat IgG and Rat Serum.
ConjugationBiotin
StorageStore vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Buffer/Preservatives0.01% (w/v) Sodium Azide
Alternative namesGoat anti-Rat IgG biotin Conjugated Antibody, Goat
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NoteFor research use only
Application notesAnti-RAT IgG Biotin Conjugated antibody has been tested by ELISA and western blot and is suitable for immunoassays where specificity to the Rat immunoglobulin heavy and or light chain regions is desired. Anti-Rat antibody has been assayed against 1.0 μg of Rat IgG in a standard capture ELISA using Peroxidase Conjugated Streptavidin and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:100,000 to 1:500,000 is suggested for this product. Optimal concentrations in immunoassays should be determined by the researcher.
Expiration Date12 months from date of receipt.
Rat IgG (H&L) Antibody Biotin Conjugated

Western Blot of Anti-Rat IgG (H&L) (GOAT) Antibody (p/n orb347799). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n orb2652744). Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgG (H&L) (GOAT) Antibody (p/n orb347799) 1:1000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.

Rat IgG (H&L) Antibody Biotin Conjugated

Western Blot of Goat anti-Rat IgG (H&L) Antibody Biotin Conjugated. Lane 1: Rat IgG. Load: 100 ng per lane. Primary antibody: Rat IgG (H&L) Antibody Biotin Conjugated at 1:1000 for 60 min RT. Secondary antibody: HRP Conjugated Streptavidin at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT.