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Catalog Number | orb347849 |
---|---|
Category | Antibodies |
Description | Rat IgG F(c) antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, IHC, WB |
Reactivity | Rat |
Isotype | IgG |
Immunogen | Rat IgG F(c) fragment |
Concentration | 2.1 mg/mL |
Dilution range | ELISA: 1:20,000 - 1:100,000, IHC: 1:1,000 - 1:5,000, WB: 1:2,000 - 1:10,000 |
Form/Appearance | Liquid (sterile filtered) |
Purity | This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against Rat IgG F(ab). |
Conjugation | Unconjugated |
Storage | Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | Rabbit Anti Rat IgG F(c) Antibody, Rabbit Anti-Rat Read more... |
Note | For research use only |
Application notes | Anti-Rat IgG F(c) antibody is tested by western blot and is suitable for ELISA and immunohistochemistry, as well as other assays requiring lot-to-lot consistency. |
Expiration Date | 12 months from date of receipt. |
Western Blot of Anti-Rat IgG F(c) (RABBIT) Antibody (p/n orb347849). Lane M: 3 μl Molecular Ladder. Lane 1: Rat IgG whole molecule. Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgG F(c) (RABBIT) Antibody (p/n orb347849) 1:1000 for 60 min at RT. Secondary Antibody: Anti-Rabbit IgG (GOAT) Peroxidase Conjugated Antibody (p/n orb347654) 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.
ELISA, IHC, SDS-PAGE, WB | |
Unconjugated | |
Rat IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Rat IgG F(c) fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat Serum, anti-Rat IgG and anti-Rat IgG F(c). No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain. | |
Rat |
HRP | |
This product was prepared from normal serum by delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti- Rat IgG, anti-Rat IgG F(c) and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain. | |
Rat |
Biotin | |
This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rat IgG, anti- Rat IgG F(c) and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain. | |
Rat |
FITC | |
This product was prepared from normal serum by delipidation, salt fractionation and ion change chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rat IgG, anti-Rat IgG F(c) and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(ab’)2 or anti-Papain. | |
Rat |
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