Rat IgG Fc Antibody Alkaline Phosphatase Conjugated

• Catalog Number: orb347815
• Category: Antibodies
• Description: Rat IgG F(c) antibody (Alkaline Phosphatase)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, IHC, WB
• Reactivity: Rat
• Isotype: IgG
• Immunogen: Rat IgG F(c) fragment
• Concentration: 1.0 mg/mL
• Dilution range: ELISA: 1:2,000 - 1:5,000, IHC: 1:200 - 1:1,000, WB: 1:500 - 1:2,500
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Alkaline Phosphatase (calf intestine), anti-Goat Serum, Rat IgG, Rat IgG F(c) and Rat Serum. No reaction was observed against Rat IgG F(ab).
• Conjugation: AP
• Storage: Store vial at 4° C before opening. DO NOT FREEZE. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. Freezing alkaline phosphatase conjugates will result in a substantial loss of enzymatic activity.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat Anti-Rat IgG F(c) alkaline phosphatase Conjug
• Note: For research use only
• Application notes: Anti-Rat F(c) IgG Alkaline Phosphatase conjugate has been tested by ELISA and dot blot and is suitable for immunoblotting (western or dot blot), ELISA, immunoelectron microscopy and immunohistochemistry as well as other antibody-based enzymatic assays requiring lot-to-lot consistency.
• Expiration Date: 12 months from date of receipt.

Western Blot of Anti-Rat IgG F(c) (GOAT) Antibody (p/n orb347800). Lane M: 3 µl Molecular Ladder. Lane 1: Rat IgG whole molecule (p/n orb2652744). Lane 2: Rat IgG F(c) Fragment (p/n orb346338). Lane 3: Rat IgG Fab Fragment (p/n orb346340). Lane 4: Rat IgM Whole Molecule (p/n orb346341). Lane 5: Rat Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rat IgG F(c) (GOAT) Antibody (p/n orb347800) 1:1000 for 60 min at RT. Secondary Antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 55 kDa for Rat IgG and Serum, 25 kDa for F(c) and Fab, 78 and 25 kDa for IgM. Rat F(c) migrates slightly higher.