Rat IgG Fab Biotin Antibody

• Catalog Number: orb346356
• Category: Proteins
• Description: Rat IgG Fab Biotin Antibody
• Isotype: IgG Fab
• Concentration: 1.0 mg/mL
• Form/Appearance: Lyophilized
• Purity: This product was prepared from normal serum delipidation, salt fractionation, ion exchange chromatography followed by papain digestion and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-Rat IgG, anti-Rat IgG F(ab’)2 and anti-Rat Serum. No reaction was observed against anti-Rat IgG F(c) or anti-Papain.
• Conjugation: Biotin
• Source: Rat
• Biological Origin: Rat
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rat IgG Fab fragment biotin conjugation, Rat IgG F
• Note: For research use only
• Application notes: Rat IgG Fab fragment reagents are ideal for ELISA, western blotting, Immunohistochemistry, as well as other antibody detection methods.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated. Lane M: 3 µL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated. Lane 3: Reduced Rat IgG Fab Fragment (p/n orb346340). Lane 4: Reduced Rat IgM Whole Molecule (p/n orb346341). Load: 1 µg of IgG, F(c), Fab; 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.