Rat IgG Antibody

• Catalog Number: orb346337
• Category: Proteins
• Description: Rat IgG Antibody
• Tested applications: ELISA, FC, SDS-PAGE, WB
• Isotype: IgG
• Concentration: 10.0 mg/mL
• Dilution range: ELISA: User Optimized, FC: User Optimized, WB: User Optimized
• Form/Appearance: Lyophilized
• Purity: Rat IgG was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rat IgG and anti-Rat Serum.
• Conjugation: Unconjugated
• Source: Rat
• Biological Origin: Rat
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Rat Immunoglobulin G, Rat IgG whole molecule, Rat
• Note: For research use only
• Application notes: Rat IgG whole molecule has been tested by SDS-Page and Western Blot and is suitable for use as antigen or ligand in immunochemical reactions, as a control or standard in assays, for conjugation and most other immunological methods requiring highly purified immunoglobulins.
• Expiration Date: 12 months from date of receipt.

SDS PAGE of Rat IgG Whole Molecule. Lane 1: Non-Reduced Rat IgG Whole Molecule. Lane 2: 5 µL Opal Prestained Marker. Lane 3: Reduced Rat IgG Whole Molecule. Load: 1 µg per lane. Predicted/Observed size: Non-Reduced at 160 kDa/Observed at 245 kDa; Reduced at 55, 25 kDa. Non-reduced IgG migrates slightly higher.

SDS-PAGE of Rat IgG F(c) Fragment Rhodamine Conjugated. Lane M: 3 µL Opal Prestained Marker. Lane 1: Reduced Rat IgG Whole Molecule. Lane 2: Reduced Rat IgG F(c) Fragment Rhodamine Conjugated. Lane 3: Reduced Rat IgG Fab Fragment (p/n orb346340). Lane 4: Reduced Rat IgM Whole Molecule (p/n orb346341). Load: 1 µg of IgG, F(c), Fab; 1.5 µg of IgM. Predicted/Observed size: IgG at 55 and 25 kDa; F(c) at 25 kDa; Fab at 25 kDa; IgM at 78 and 25 kDa. Observed F(c) Fragment migrates slightly higher.