Rat HB ELISA Kit

• Catalog Number: orb781016
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hemoglobin(HB) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hemoglobin(HB).
• Tested applications: ELISA
• Reactivity: Rat
• Range: 7.82-500 µg/mL
• Concentration: 500 µg/mL
• Sensitivity: 2.56 µg/mL
• Protein Length: 2h
• Alternative names: Hgb ELISA Kit, Haemoglobin ELISA Kit, Heterotetram
• Note: For research use only
• Application notes: standard: 500 µg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat HB protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat HB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat HB in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: serum, plasma, tissue homogenates, erythrocyte lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.