Rat Glucagon (GC) ELISA Kit

• Catalog Number: orb780110
• Category: Assays and Kits
• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Glucagon(GC) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Glucagon(GC).
• Tested applications: ELISA
• Reactivity: Rat
• Range: 23.44-1500 pg/mL
• Assay Time: 2.5h
• Concentration: 1500 pg/mL
• UniProt ID: P06883
• Sensitivity: 8.99 pg/mL
• Alternative names: GCG ELISA Kit, GLP1 ELISA Kit, GLP2 ELISA Kit, GRP
• Note: For research use only
• Application notes: standard: 1500 pg/mL. Test principle: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat GC protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GC. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GC in the samples is then determined by comparing the OD of the samples to the standard curve
• Sample Types: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Expiration Date: Please enquire.