Rat Complement C5 Protein

• Catalog Number: orb570266
• Category: Proteins
• Description: Rat Complement C5, His Tag is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Ala 1688 (Accession # XP_001079130.2).
• Reactivity: Rat
• Tag: C-10×His
• Form/Appearance: Powder
• Purity: 95%
• Conjugation: Unconjugated
• MW: 74.1 kDa (β chain) & 114.8 kDa (α chain)
• Target: Complement C5
• Source: Rat Complement C5, His Tag (orb570266) is expressed from human 293 cells (HEK293). It contains AA Gln 24 - Ala 1688 (Accession # XP_001079130.2).
• Expression System: HEK293
• Biological Origin: Rat
• Biological Activity: Immobilized Rat Complement C5, His Tag at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-12.5 ng/mL (QC tested).
• Expression Region: Gln 24 - Ala 1688
• Endotoxins: 1.0 EU per μg
• NCBI: XP_001079130.2
• Storage: -20°C to -70°C for 12 months in lyophilized state; -70°C for 3 months under sterile conditions after reconstitution. For long term storage, the product should be stored at lyophilized state at -20°C or lower.
• Buffer/Preservatives: PBS, pH7.4
• Alternative names: Complement C5,C5,CPAMD4
• Note: For research use only
• Application notes: This protein carries a polyhistidine tag at the C-terminus. The mature form of Complement C5 is a disulfide-linked heterodimer composed of proteolytically cleaved α and β chain. Each α and β chain has a calculated MW of 74.1 kDa (β chain) and 114.8 kDa (α chain). The protein migrates as 70 kDa and 110 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
• Expiration Date: 6 months from date of receipt.

Immobilized Rat Complement C5, His Tag (Cat. No. orb570266) at 2 μg/mL (100 μL/well) can bind Anti-C5a (Human IgG1) with a linear range of 0.4-12.5 ng/mL (QC tested).