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Catalog Number | orb766203 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody to RAR beta |
Clonality | Polyclonal |
Tested applications | ELISA, IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | IgG |
Immunogen | The antiserum was produced against synthesized peptide derived from human Retinoic Acid Receptor beta. AA range:331-380 |
Concentration | 1 mg/ml |
Dilution range | Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications. |
Conjugation | Unconjugated |
MW | 50 |
Source | Rabbit |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
Buffer/Preservatives | PBS with 0.02% sodium azide and 50% glycerol pH 7.4. |
Alternative names | Anti-RARB antibody, anti-HAP antibody, anti-NR1B2 Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot analysis of various cells using RARβ Polyclonal Antibody.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using Retinoic Acid Receptor beta Antibody. The picture on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HepG2 cells, using Retinoic Acid Receptor beta Antibody. The lane on the right is blocked with the synthesized peptide.
Western blot analysis of lysates from HepG2, Hela cells, (Green) primary antibody was diluted at 1:1000, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour. (Red) loading contrl antibody was diluted at 1:5000 as loading control, 4°C over night, secondary antibody was diluted at 1:10000, 37°C 1 hour.