RANKL/CD254 Rabbit Polyclonal Antibody

• Catalog Number: orb500861
• Category: Antibodies
• Description: RANKL/CD254 Rabbit Polyclonal Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, WB
• Predicted Reactivity: Bovine, Canine, Equine, Porcine, Rabbit, Zebrafish
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RANKL/CD254 (41-140/317aa)
• Concentration: 1mg/ml
• Dilution range: WB=1:500-2000, Flow-Cyt=1ug/Test
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 35 kDa
• Target: TNFSF11
• UniProt ID: O14788
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: OPGL; CD254; hRANKL2; ODF; OPGL; Osteoclast differ
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Blank control: HL-60. Primary Antibody (green line): Rabbit Anti-RANKL/CD254 antibody (orb500861), dilution: 1 µg/10^6 cells, Isotype Control Antibody (orange line): Rabbit IgG. Secondary Antibody: Goat anti-rabbit IgG-AF488, dilution: 1 µg/Test. Protocol, The cells were fixed with 4% PFA (10 min at room temperature) and then permeabilized with 0.1% PBST for 20 min at room temperature. The cells were then incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-CD254 (orb500861) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 35 kD, Observed band size: 37/50 kD.

Sample: Lymph node (Mouse) Lysate at 40 ug, Thymus (Mouse) Lysate at 40 ug, Lymph node (Rat) Lysate at 40 ug, Primary: Anti-RANKL'CD254 (orb500861) at 1/500 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37'50 kD, Observed band size: 50 kD.