RANK Rabbit Polyclonal Antibody

SKU: orb312842

Description

RANK Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: FC, ICC
• Dilution range: ICC/IF=1:50-100, Flow-Cyt=1μg /test
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human RANK (51-150/616aa)
• Target: TNFRSF11A
• Molecular Weight: 82-90 kDa
• Purification: Affinity purified by Protein A

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Disclaimer: For research use only

Alternative Names

TNFRSF11A; CD265; CD265 antigen; Activator of NFKB; EOF; FEO; mRANK; NFKB activator; ODFR; OFE; Osteoclast differentiation factor receptor; PDB 2; Receptor activator of NF KB; receptor activator of nuclear factor kappa B; TNFRSF 11A; TNFSF11; TRANCE R; RANK receptor; TNR11_HUMAN.

Blank control (blue): Jurkat cells (fixed with 2% paraformaldehyde (10 min)). Primary Antibody: Rabbit Anti-RANK antibody (orb312842), dilution: 1 µg in 100 µl 1X PBS containing 0.5% BSA, Isotype Control Antibody: Rabbit IgG (orange), used under the same conditions, Secondary Antibody: Goat anti-rabbit IgG-PE (white blue), dilution: 1:200 in 1X PBS containing 0.5% BSA.

Sample: Lung (Mouse) Lysate at 40 ug, Primary: Anti-RANK (orb312842) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63-70 kD, Observed band size: 70 kD.

Sample: U251 Cell Lysate at 30 ug, Primary: Anti-RANK (orb312842) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 63-70 kD, Observed band size: 63 kD.

Quick Database Links

Gene Symbol

TNFRSF11A

UniProt

Q9Y6Q6