Raf1 Antibody

• Catalog Number: orb1273566
• Category: Antibodies
• Description: Raf1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: WB
• Reactivity: Bovine, Canine, Frog, Gallus, Human, Mouse, Rat
• Immunogen: Phosphopeptide corresponding to amino acid residues surrounding the phospho-Ser301 of rat Raf-1.
• Concentration: batch dependent
• Dilution range: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 74
• Target: Raf1
• UniProt ID: P11345
• NCBI: P11345
• Storage: Raf-1 antibody can be stored at -20°C and is stable at -20°C for at least 1 year.
• Note: For research use only
• Application notes: The antibody has been directly tested for reactivity in Western blots with rat tissue. It is anticipated that the antibody will react with bovine, canine, chicken, human, mouse, non-human primate and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.
• Expiration Date: 12 months from date of receipt.

Western blot of UV treated human Jurkat cell lysate showing specific immunolabeling of the ~74k Raf-1 protein (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase: lamda-Ptase). The blot is identical to the control except that it was incubated in lamda-Ptase (1200 units for 30 min) before being exposed to the phospho Ser301 Raf-1 antibody. The immunolabeling of Raf-1 is completely eliminated by treatment with lamda-Ptase.