Raf1 Antibody

• Catalog Number: orb1268426
• Category: Antibodies
• Description: Raf1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC-P, WB
• Predicted Reactivity: Rat
• Reactivity: Human, Mouse
• Isotype: Rabbit Ig
• Immunogen: This Mouse Raf1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 86-113 amino acids from the N-terminal region of mouse Raf1.
• Concentration: batch dependent
• Dilution range: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 73 kDa
• Target: Raf1
• UniProt ID: Q99N57
• NCBI: Q99N57
• Storage: Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: Supplied in PBS with 0.09% (W/V) sodium azide.
• Alternative names: RAF proto-oncogene serine/threonine-protein kinase
• Note: For research use only
• Application notes: For WB starting dilution is: 1:1000For FACS starting dilution is: 1:10~50For IHC-P starting dilution is: 1:10~50
• Expiration Date: 12 months from date of receipt.

Western blot analysis in Jurkat cell line lysates (35 ug/lane).

Western blot analysis in mouse Neuro-2a cell line lysates (35 ug/lane).

Flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.

Mouse Raf1 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded mouse skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.