Rad51 Recombinant Rabbit Monoclonal Antibody

• Catalog Number: orb608069
• Category: Antibodies
• Description: Rad51 Recombinant Rabbit Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: 11D1
• Tested applications: IF, IHC-Fr, IHC-P, WB
• Predicted Reactivity: Mouse, Rat
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Rad51
• Concentration: 1mg/ml
• Dilution range: WB=1:500, IHC-P=1:200-500, IHC-F=1:200-500, IF=1:200-500
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 37 kDa
• Target: RAD51
• UniProt ID: Q06609
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Alternative names: BRCA1/BRCA2 containing complex, subunit 5; BRCC 5;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Paraformaldehyde-fixed, paraffin embedded Human Breast Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated (orb608069) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon Cancer, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated (orb608069) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated (orb608069) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Testicles, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated (orb608069) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Human Tonsil, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated (orb608069) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Humann Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated (orb608069) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated (orb608069) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated (orb608069) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Mouse Testicles, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated (orb608069) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Colon, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated (orb608069) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Kidney, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated (orb608069) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Paraformaldehyde-fixed, paraffin embedded Rat Testicles, Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Antibody incubation with Rad51 Monoclonal Antibody, Unconjugated (orb608069) at 1:200 overnight at 4°C, followed by conjugation to the SP Kit (Rabbit) and DAB staining.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Thymus tissue lysates, Lane 3: Human HeLa cell lysates, Lane 4: Human Jurkat cell lysates, Primary: Anti-Rad51 (orb608069) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 37 kDa, Observed band size: 36 kDa.