RAD50 Antibody

• Catalog Number: orb1257961
• Category: Antibodies
• Description: RAD50 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, IP, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RAD50 (NP_005723.2).
• Concentration: batch dependent
• Dilution range: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:20 - 1:50
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Observed: 170kDa
• Target: RAD50
• UniProt ID: Q92878
• Storage: Store at -20˚°C. Avoid freeze / thaw cycles.
• Buffer/Preservatives: Liquid
• Alternative names: DNA repair protein RAD50, hRAD50, NBSLD, RAD502, h
• Note: For research use only
• Application notes: WB: 1:500 - 1:2000IF: 1:50 - 1:200IP: 1:20 - 1:50
• Expiration Date: 12 months from date of receipt.

Western blot analysis of extracts of various cell lines, using RAD50 antibody (orb1257961) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunofluorescence analysis of U2OS cells using RAD50 antibody (orb1257961) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation analysis of 150 ug extracts of MCF7 cells using 3 ug RAD50 antibody (orb1257961). Western blot was performed from the immunoprecipitate using RAD50 antibody (orb1257961) at a dilution of 1:500.