You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1672055 |
---|---|
Category | Antibodies |
Description | RAD23B Antibody |
Clonality | Recombinant |
Clone Number | SAIC-28B-8 |
Reactivity | Human |
Isotype | IgG kappa |
Immunogen | Peptide "IDIDPEETVK", derived from the UV excision repair protein RAD23 homolog B, conjugated to KLH. |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | RAD23B |
UniProt ID | P54727 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | HHR23B, HR23B, P58, RAD23 homolog B, nucleotide ex Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence staining of HeLa cells with anti-RAD23B (SAIC-28B-8). Immunofluorescence analysis of paraformaldehyde fixed HeLa cells permewith 0.15% Triton stained with the chimeric r version of SAIC-28B-8 (orb1672055) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution)- showing nuclear and cytoplasmic staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Panels show from left-right- top-bottom orb1672055- DAPI- merged channels and an isotype control. The isotype control was an unknown specificity antibody (3.0) followed by staining with Alexa Fluor® 488 secondary antibody.
Flow cytometry using the Anti-RAD23B antibody SAIC-28B-8. Paraformaldehyde fixed HeLa cells permewith 0.5% Triton were stained with anti-unknown specificity antibody (3.0; isotype control - black line) or the r version of SAIC-28B-8 (orb1672055 - blue line) at a dilution of 1:100 for 1h at RT. After washing- the bound antibody was detected using a goat anti-r AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
Western Blot using anti-RAD23B antibody SAIC-28B-8. Nuclear lysate of MCF7 cells (35 ug protein in RIPA buffer) were resolved on a SDS PAGE gel and blots were probed with the chimeric rsion of SAIC-28B-8 (orb1672055) at 2 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
ICC, IHC-Fr, IHC-P, WB | |
Hamster, Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
Filter by Rating