RACGAP1 Antibody

• Catalog Number: orb1247045
• Category: Antibodies
• Description: RACGAP1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: C-GRQGNFFASPMLK
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell line Jurkat, and in nuclear lysates of cell lines A431 and Jurkat (calculated MW of 71.0kDa according to NP_037409.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.Immunohistochemistry:In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration: 1ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: RACGAP1
• UniProt ID: Q9H0H5
• NCBI: NP_037409.2, NP_001306935.1, NP_001306936.1, NP_001306934.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RACGAP1, MgcRacGAP, Rac GTPase activating protein
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 75kDa band observed in lysates of cell line Jurkat, and in nuclear lysates of cell lines A431 and Jurkat (calculated MW of 71.0kDa according to NP_037409.2). Recommended concentration: 0.3-1ug/ml. Primary incubation 1 hour at room temperature. Negative Control: Human Pancreas lysate.Immunohistochemistry:In paraffin embedded Human Testis, shows nuclear staining of many cells. Recommended concentration: 1ug/ml.Immunofluorescence: Strong expression of the protein seen in the cytoplasm of MCF7 cells. Recommended concentration: 10ug/ml. Flow Cytometry: Flow cytometric analysis of MCF7 cells. Recommended concentration: 10ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247045 (4 ug/ml) staining of paraffin embedded Human Testis. Microwaved antigen retrieval with Tris/EDTA buffer pH9, HRP-staining.

orb1247045 (1 ug/ml) staining of A431 nuclear (A), Jurkat (B), Jurkat nuclear (C) and negative control Human Pancreas (D) lysate. (35 ug protein in RIPA buffer) Detected by chemiluminescence.

orb1247045 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml), showing nuclear staining. The nuclear stain is DAPI (blue).

orb1247045 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488 secondary antibody (4 ug/ml). IgG control: Unimmunized goat IgG (black line).