RABBIT IgG (H&L) antibody

• Catalog Number: orb347649
• Category: Antibodies
• Description: RABBIT IgG (H&L) antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Rabbit
• Isotype: IgG
• Immunogen: Anti-Rabbit IgG (H&L) was produced by repeated immunization with rabbit whole IgG molecule in goat.
• Concentration: 1.167 mg/mL
• Dilution range: ELISA: 1:15,000 - 1:30,000, IHC: 1:500 - 1:2,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG and Rabbit Serum. No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat and Sheep Serum Proteins. Specificity was confirmed using ELISA against at less than 1% of target signal.
• Conjugation: Unconjugated
• Storage: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-Rabbit IgG Antibody, goat anti-Rabbit Ig
• Note: For research use only
• Application notes: Anti-Rabbit IgG (H&L) has been tested by ELISA and western blot and is suitable for use in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Specific conditions for reactivity and signal detection should be optimized by the end user.
• Expiration Date: 12 months from date of receipt.

SDS of Goat Anti-Rabbit IgG Antibody Min X 10. Lane 1: Nonreduced Rabbit IgG whole molecule. Molecular Ladder. Lane 2: Reduced Rabbit IgG whole molecule. 4-20% SDS Gel, Coomassie Blue Stained.

Western Blot of Goat anti-RABBIT IgG (H&L) Antibody Pre-adsorbed. Lane 1: Rabbit IgG. Load: 100 ng per lane. Primary antibody: RABBIT IgG (H&L) Antibody Pre-adsorbed at 1:1000 for overnight at 4°C. Secondary antibody: DyLight™ 800 goat secondary antibody 1:20000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 55 and 28 kDa, 55 and 28 kDa for Rabbit IgG.

Western Blot of Unconjugated Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n orb347649). Lane M: 3 μl Molecular Ladder. Lane 1: Rabbit IgG whole molecule. Lane 2: Rabbit IgG F(ab) Fragment. Lane 3: Rabbit IgG F(c) Fragment (p/n orb346280). Lane 4: Rabbit IgM Whole Molecule (p/n orb346313). Lane 5: Normal Rabbit Serum. All samples were reduced. Load: 50 ng per lane. Block: orb348637 for 30 min at RT. Primary Antibody: Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n orb347649) 1:1000 for 60 min at RT. Secondary antibody: Anti-Goat IgG (DONKEY) Peroxidase Conjugated Antibody 1:40000 in orb348637 for 30 min at RT. Predicted/Observed Size: 25 and 50 kDa for Rabbit IgG and Serum, 25 kDa for F(c) and F(ab), 70 and 23 kDa for IgM. Rabbit F(c) migrates slightly higher.