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Catalog Number | orb752331 |
---|---|
Category | Proteins |
Description | Rabbit IgG Fc, Tag Free is expressed from human 293 cells (HEK293). It contains AA Cys 105 - Lys 323 (Accession # P01870-1). |
Reactivity | Rabbit |
Tag | Native |
Form/Appearance | Powder |
Purity | 95% |
Conjugation | Unconjugated |
MW | 24.8 kDa |
Target | IgG Fc |
UniProt ID | P01870 |
Protein Sequence | Cys 105 - Lys 323 |
Source | Rabbit IgG Fc, Tag Free (orb752331) is expressed from human 293 cells (HEK293). It contains AA Cys 105 - Lys 323 (Accession # P01870-1). |
Expression System | HEK293 |
Biological Origin | Rabbit |
Expression Region | Cys 105 - Lys 323 |
Endotoxins | 1.0 EU per μg |
Storage | -20℃ |
Buffer/Preservatives | 50 mM Tris, 100 mM Glycine, 25 mM Arginine, 150 mM NaCl, pH7.5 |
Alternative names | IgG Fc Read more... |
Note | For research use only |
Application notes | This protein carries no "tag". The protein has a calculated MW of 24.8 kDa. The protein migrates as 28-32 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation. |
Expiration Date | 6 months from date of receipt. |
ELISA, IHC, SDS-PAGE, WB | |
Unconjugated | |
Rabbit IgG F(c) fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Rabbit IgG F(c) fragment assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Rabbit IgG and anti-Rabbit IgG F(c). No reaction was observed against anti-Rabbit IgG F(ab’)2 or anti-Papain. | |
Rabbit |
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