Rabbit IgG Fc Antibody Rhodamine Conjugated

• Catalog Number: orb347647
• Category: Antibodies
• Description: Rabbit IgG F(c) antibody (TRITC)
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: FC, FLISA, IF
• Reactivity: Rabbit
• Isotype: IgG
• Immunogen: Rabbit IgG F(c) fragment
• Concentration: 2.0 mg/mL
• Dilution range: FLISA: 1:10,000 - 1:50,000, FC: 1:500 - 1:2,500, IF: 1:1,000 - 1:5,000
• Form/Appearance: Lyophilized
• Purity: This product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Rabbit IgG, Rabbit IgG F(c) and Rabbit Serum. No reaction was observed against Rabbit IgG F(ab).
• Conjugation: TRITC
• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Goat anti-Rabbit IgG F(c) Antibody Rhodamine Conju
• Note: For research use only
• Application notes: This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
• Expiration Date: 12 months from date of receipt.

(a) Channels coating: remnant fluorescence due to nonspecific binding of anti-rabbit IgG labeled with B-rhodamine (p/n orb347647) [AbII] at a concentration of 100 µg/ml in uncoated and coated microchannels with silane-PEG. (b) Fluorescence obtained from the complete immunoassay at a flow rate of 5 µl/h and at a anti-biotin rabbit IgG (p/n orb750609) [AbI] concentration of 50 pg/ml is compared to the fluorescence obtained from the immunoassay performed without applying the magnetic field (column 1), without adding anti-biotin rabbit IgG (p/n orb750609) [AbI] (column 2), without adding), anti-rabbit IgG labeled with B-rhodamine (p/n orb347647) [AbII] (column 3), and, finally, the efficacy of the microparticles coating was tested by performing the immunoassay without anti-biotin rabbit IgG (p/n orb750609) [AbI] and with noncoated microparticles (column 5). The level of fluorescence of the two first columns in b results from nonspecific interactions of anti-rabbit IgG labeled with B-rhodamine (p/n orb347647) [AbII] with the microparticles. Error bars are standard deviation.