Rabbit IgG Fab Antibody

• Catalog Number: orb346312
• Category: Proteins
• Description: Rabbit IgG Fab Antibody
• Tested applications: ELISA, IHC, SDS-PAGE, WB
• Isotype: IgG Fab
• Concentration: 2.0 mg/mL
• Dilution range: ELISA: User Optimized, IHC: User Optimized, WB: User Optimized
• Form/Appearance: Liquid (sterile filtered)
• Purity: Rabbit IgG Fab fragment was prepared from normal serum by a multi-step process which includes delipidation, salt fractionation, ion exchange chromatography and papain digestion followed by chromatographic separation and extensive dialysis against the buffer stated above. Rabbit IgG Fab fragment was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum, anti-Rabbit IgG and anti-Rabbit IgG F(ab’)2. No reaction was observed against anti-Rabbit IgG F(c) or anti-Papain.
• Conjugation: Unconjugated
• Source: Rabbit
• Biological Origin: Rabbit
• Storage: Store vial at 4° C prior to opening. This product is stable 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide. 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Alternative names: Rabbit Immunoglobulin Fab, F(ab), Fragment antigen
• Note: For research use only
• Application notes: Rabbit IgG Fab Fragment has been tested in SDS-Page and can be utilized as a control or standard reagent in Western Blotting and ELISA experiments.
• Expiration Date: 12 months from date of receipt.

SDS-PAGE of Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane M: 3 µL Opal Prestained Marker. Lane 1: Reduced Rabbit IgG Whole Molecule Rhodamine Conjugated (p/n orb346302). Lane 2: Reduced Rabbit IgG F(ab) Fragment. Lane 3: Reduced Rabbit IgG F(c) Fragment (p/n orb346310). Lane 4: Reduced Rabbit IgM Whole Molecule (p/n orb346313). Load: 1 µg for F(ab) and F(c); 1.2 µg for IgG and IgM. Predicted/Observed size: IgG at 50 and 25 kDa; F(c) at 25 kDa; F(ab) at 25 kDa; IgM at 70 and 23 kDa. Observed F(c) Fragment migrates slightly higher.