Rabbit IgG Antibody

• Catalog Number: orb1563302
• Category: Antibodies
• Description: Rabbit IgG Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5B2-F9-D6
• Tested applications: ELISA, WB
• Reactivity: Rabbit
• Isotype: IgG1
• Immunogen: Rabbit IgG
• Concentration: 5.85 mg/ml
• Dilution range: WB: 1/500-1/1000 ELISA: 1/10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Calculated MW: 55,25 kDa; Observed MW: 55,25 kDa
• Target: Rabbit IgG
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
• Alternative names: Antibody; Ig gamma chain C region; Ig kappa-b4 cha
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of MEK2 in THP-1, Jurkat, Raji, 3T3 and Hela lysates using MEK2 antibody, then incubated with orb1563302 (Rabbit IgG mAb).Anti-mouse IgG, HRPlinked antibody was used for detecting.

Western blot analysis of Rabbit IgG (5B2) in decreasing concentrations of total rabbit IgG reduced and denatured in 2X SDS loading buffer with DTT using IgG (HeavyChain Specific) antibody.

Immunoprecipitation analysis of Rabbit IgG (5B2) in CHO-K1 lysates transfected with HAtagged NGAL using HATag antibody (lane 2). Lane 1 contains rabbit IgG IP control lysates. All lanes are probed with HATag antibody as the primary antibody. Panel A incubated with secondary antibodies anti-rabbit whole IgG, HRPlinked antibody for detecting.Panel B incubated with anti-rabbit IgG (HeavyChain Specific) mAb and the Anti-mouse whole IgG, HRPlinked antibody was used for detecting.