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| Catalog Number | orb1946619 |
|---|---|
| Category | Assays and Kits |
| Description | Rabbit Cr(Calretinin) ELISA Kit |
| Reactivity | Rabbit |
| Range | 1.25-80 nmol/ml |
| Target | CR |
| Sensitivity | 0.75 nmol/ml |
| Alternative names | CR; Calretinin; CALB2; CAB29; CAL2; 29 kDa calbind Read more... |
| Note | For research use only |
| Application notes | This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Calretinin(Cr) protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Calretinin(Cr). Next,Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Calretinin(Cr) in the samples is then determined by comparing the OD of the samples to the standard curve. |
| Sample Types | Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids. |
| Expiration Date | Please enquire. |
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