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Catalog Number | orb1526639 |
---|---|
Category | Antibodies |
Description | WDR5 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP |
Reactivity | Human |
Immunogen | Between 1 and 50 |
Antibody Type | Primary Antibody |
Concentration | 1000 µg/ml |
Dilution range | WB - Not recommended. Use rabbit anti-WDR5 antibody A302-430A.; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | WDR5 |
UniProt ID | P61964 |
NCBI | NP_060058.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | WD repeat-containing protein 5; BIG-3; BMP2-induce Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human WDR5 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-WDR5 antibody orb1526639 used for IP at 6 µg per reaction.
Detection of human WDR5 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-WDR5 (Cat. No. orb1526639) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ChIP, ELISA, FC, IF | |
Human | |
Recombinant | |
Unconjugated |