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Catalog Number | orb1519607 |
---|---|
Category | Antibodies |
Description | Tyrosine Hydroxylase Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Clone Number | BLR155J |
Tested applications | ICC, IHC, WB |
Reactivity | Human, Mouse |
Immunogen | Between 478 and C-terminus |
Concentration | 250 µg/ml |
Dilution range | WB - 1:1,000; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | Tyrosine Hydroxylase |
UniProt ID | P07101 |
NCBI | NP_954986.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Phosphate Buffered Saline (PBS) containing 0.2% rAlbumin and 0.09% Sodium Azide |
Alternative names | TYH; tyrosine 3-hydroxylase; tyrosine 3-monooxygen Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of mouse Tyrosine Hydroxylase by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, ND7/23, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Tyrosine Hydroxylase recombinant monoclonal antibody (orb1519607) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody.
ICC, IHC, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
ICC, IHC, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |