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Catalog Number | orb1523878 |
---|---|
Category | Antibodies |
Description | SNRPA1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 205 and C-terminus |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | SNRPA1 |
UniProt ID | P09661 |
NCBI | NP_003081.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | Lea1; U2 small nuclear ribonucleoprotein A'; U2 sm Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse SNRPA1 by western blot. Samples: Whole cell lysate (50 µg) from Jurkat, HEK293T, HeLa, mouse TCMK-1, and mouse NIH 3T3 cells. Antibodies: Affinity purified rabbit anti-SNRPA1 antibody orb1523878 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human SNRPA1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified rabbit anti-SNRPA1 antibody orb1523878 used for IP at 6 µg/mg lysate.
Detection of human SNRPA1 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-SNRPA1 (Cat. No. orb1523878) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB
Detection of human SNRPA1 by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-SNRPA1 (Cat. No. orb1523878) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB
WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |