Rabbit anti-SNRNP200 Antibody, Affinity Purified

• Catalog Number: orb1524807
• Category: Antibodies
• Description: SNRNP200 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 400 and 450
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 - 1:2,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SNRNP200
• UniProt ID: O75643
• NCBI: NP_054733.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: BRR2 homolog; small nuclear ribonucleoprotein 200k
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human SNRNP200 by western blot. Samples: Whole cell lysate (10 µg) from HEK293T, HeLa, K-562, Jurkat, and RKO cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SNRNP200 antibody (orb1524807) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of mouse SNRNP200 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-SNRNP200 antibody (orb1524807) used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Detection of human SNRNP200 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-SNRNP200 antibody (orb1524807) used for IP at 6 µg per reaction. SNRNP200 was also immunoprecipitated by a previous lot of this antibody (orb1524807) and a second antibody against a different epitope of SNRNP200.

Detection of human SNRNP200 by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- SNRNP200 (Cat. No. orb1524807) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB. Counterstain: hematoxylin (blue).