Rabbit anti-SMC1 Recombinant Monoclonal Antibody

• Catalog Number: orb1519958
• Category: Antibodies
• Description: SMC1 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BL-205-2G8
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 1183 and C-terminus
• Concentration: 50 µg/ml
• Dilution range: WB - 1:1000; IP - 20 µl/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 - 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SMC1
• UniProt ID: Q14683
• NCBI: NP_006297.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: SMC protein 1A; SMC-1A; structural maintenance of
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse SMC1 by western blot. Samples: Whole cell lysate (50 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Detection of human SMC1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, MCF-7, Jurkat, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human SMC1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse SMC1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse SMC1 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (orb1519958) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human SMC1 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-SMC1 recombinant monoclonal [BL-205-2G8] (orb1519958) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human SMC1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958) used for IP at 20 µl/mg lysate.

Detection of human SMC1 by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human SMC1 in FFPE breast carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-SMC1 recombinant monoclonal antibody [BL-205-2G8] (orb1519958). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI.