Rabbit anti-SF3B3 Antibody, Affinity Purified

• Catalog Number: orb1526495
• Category: Antibodies
• Description: SF3B3 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 1167 and C-terminus
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SF3B3
• UniProt ID: Q15393
• NCBI: NP_036558.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: SF3b130; STAF130; spliceosome-associated protein 1
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human and mouse SF3B3 by western blot (h&m) and immunoprecipitation (h). Samples: Whole cell lysate from HeLa (5, 15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , HEK293T (T; 50 µg) , and mouse NIH 3T3 (M; 50 µg) cells. Antibodies: Affinity purified rabbit anti-SF3B3 antibody orb1526495 used for WB at 0.04 µg/ml (A) and 0.1 µg/ml (B) and used for IP at 3 µg/mg lysate. Detection: Chemiluminescence with exposure times of 1 second (A and B).

Detection of human and mouse SF3B3 by immunohistochemistry.Sample: FFPE sections of human prostate carcinoma (left) and mouse teratoma (right). Antibody: Affinity purified rabbit anti-SF3B3 (Cat. No. orb1526495) used at a dilution of 1: 200 (1 µg/ml). Detection: DAB