Rabbit anti-RAN Antibody

• Catalog Number: orb1523255
• Category: Antibodies
• Description: RAN Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 166 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:10,000 - 1:25,000; IP - 2 - 10 µg/mg lysate; IHC - 1:500 to 1:2,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RAN
• UniProt ID: P62826
• NCBI: NP_006316.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: OK/SW-cl.81; TC4; ras-related nuclear protein; Ran
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse RAN by western blot. Samples: Whole cell lysate (2 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAN antibody (orb1523255) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human RAN by western blot. Samples: Whole cell lysate (2 µg) from HeLa, Hep-G2, Jurkat, HEK293T, and U2OS cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RAN antibody (orb1523255) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.

Detection of human RAN by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RAN antibody (orb1523255) used for IP at 6 µg per reaction. RAN was also immunoprecipitated by a previous lot of this antibody (orb1523255) and a another antibody against a different epitope of RAN.

Detection of human RAN by immunohistochemistry. Samples: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-RAN (orb1523255) used at a dilution of 1: 1, 000 (1ug/ml). Detection: DAB

Detection of human RAN by immunohistochemistry. Samples: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-RAN (orb1523255) used at a dilution of 1: 5, 000 (0.2ug/ml). Detection: DAB