Rabbit anti-RALY Antibody, Affinity Purified

• Catalog Number: orb1527277
• Category: Antibodies
• Description: RALY Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 256 and C-terminus
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - Not recommended. Use rabbit anti-RALY antibody A302-069A.; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RALY
• UniProt ID: Q9UKM9
• NCBI: NP_057951.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: hnRNP associated with lethal yellow protein homolo
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human RALY by western blot. Samples: Whole cell lysate (10 µg) from Jurkat, HEK293T, Hep-G2, U2OS, and HeLa cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-RALY antibody (orb1527277) used for WB at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.

Detection of human RALY by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from U2OS cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-RALY antibody (orb1527277) used for IP at 6 µg per reaction. RALY was also immunoprecipitated by a previous lot of this antibody (orb1527277) and a second antibody against a different epitope of RALY.

Detection of mouse RALY by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti- RALY (orb1527277) used at a dilution of 1: 1, 000 (0.2 µg/ml).

Detection of human RALY by immunohistochemistry.Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- RALY (orb1527277) used at a dilution of 1: 1, 000 (0.2 µg/ml).