Rabbit anti-Phospho SMC1 (S966) Antibody

• Catalog Number: orb1530759
• Category: Antibodies
• Description: SMC1, Phospho (S966) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human
• Immunogen: Surrounding serine 966
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:1,000 to 1:5,000. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:500 - 1:5,000. Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: SMC1, Phospho (S966)
• UniProt ID: Q14683
• NCBI: NP_006297.2
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: SMC protein 1A; SMC-1A; structural maintenance of
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of Phospho SMC1 (S966) by western blot.Samples: Jurkat cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S966) antibody orb1530759 used at 0.1 µg/ml.

Detection of human Phospho SMC1 (S966) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S966) used at a dilution of 1:1, 000 (1µg/ml).

Detection of human Phospho SMC1 (S966) by immunohistochemistry. Sample: FFPE section of human testicular seminoma. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S966) used at a dilution of 1:1, 000 (1µg/ml).

Detection of human Phospho SMC1 (S966) by western blot of immunoprecipitates.Samples: Whole cell lysate from Jurkat cells treated with 100 µM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho SMC1 (S966) antibody.

Detection of human Phospho SMC1 (S966) by ICC.Samples: HeLa cells. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S966) used at a dilution of 1:5, 000. Detection: Red fluorescent Anti-rabbit IgG-DyLight® 594 conjugated.