Rabbit anti-Mre11 Antibody

• Catalog Number: orb1523782
• Category: Antibodies
• Description: Mre11 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: FC, IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 658 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Mre11
• UniProt ID: P49959
• NCBI: NP_005582.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: MRE11B; MRE11 homolog 1; MRE11 homolog A; MRE11 ho
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Mre11 by western blot. Samples: Whole cell lysate (50 µg) from HEK293T, HeLa, and Jurkat cells. Antibodies: Affinity purified rabbit anti-Mre11 antibody orb1523782 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.

Detection of human Mre11 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HEK293T cells. Antibodies: Affinity purified rabbit anti-Mre11 antibody orb1523782 used for IP at 6 µg/mg lysate.

Detection of human Mre11 by immunohistochemistry.Sample: FFPE section of human stomach linitis plastica. Antibody: Affinity purified rabbit anti-Mre11 (Cat. No. orb1523782) used at a dilution of 1: 1, 000 (1µg/ml). Detection: DAB

Detection of human Mre11 (shaded) in Jurkat cells by flow cytometry.Antibody: Rabbit anti-Mre11 polyclonal antibody (orb1523782) or isotype control (unshaded). Secondary: DyLight® 488-conjugated goat anti-rabbit IgG.