Rabbit anti-KLF5 Recombinant Monoclonal Antibody

• Catalog Number: orb1733252
• Category: Antibodies
• Description: KLF5 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR243L
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 325 and 375
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: KLF5
• UniProt ID: Q13887
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: GC-box-binding protein 2; Kruppel-like factor 5 (i
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of mouse KLF5 by western blot. Samples: Whole cell lysate (25 µg) from CT26, WEHI-231, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human KLF5 by western blot. Samples: Whole cell lysate (25 µg) from HeLa, SW620, MCF-7, and KM12 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse KLF5 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human KLF5 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human KLF5 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of mouse KLF5 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human KLF5 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from KM12 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252) used for IP at 6 µl/mg lysate.

Detection of human KLF5 by immunocytochemistry. Sample: FFPE section of HT-29 cells. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse KLF5 by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-KLF5 recombinant monoclonal antibody (orb1733252). Secondary: HRP-conjugated goat anti-rabbit IgG.