Rabbit anti-KLF4 Recombinant Monoclonal Antibody [BLR250L]

• Catalog Number: orb1710839
• Category: Antibodies
• Description: KLF4 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 75 and 125
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1,000, IP: 6 µl/1 mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections., ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: KLF4
• UniProt ID: O43474
• Storage: Shelf life: 1 year from date of receipt. Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: endothelial Kruppel-like zinc finger protein; Krup
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human KLF4 by western blot. Samples: Whole cell lysate (25 µg) from DLD-1, PC-3, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse KLF4 by western blot. Samples: Whole cell lysate (25 µg) from CH27, CT26, C2C12, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse KLF4 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human KLF4 by immunohistochemistry. Sample: FFPE section of human gastric carcinoma. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human KLF4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF-7 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839) used for IP at 6 µl/mg lysate.

Detection of human KLF4 by immunocytochemistry. Sample: FFPE section of HCT 116 cells. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse KLF4 by immunocytochemistry. Sample: FFPE section of CT26 cells. Antibody: Rabbit anti-KLF4 recombinant monoclonal antibody (orb1710839). Secondary: HRP-conjugated goat anti-rabbit IgG.