Rabbit anti-Ki-67 Recombinant Monoclonal Antibody [BLR021E]

• Catalog Number: orb1519949
• Category: Antibodies
• Description: Ki-67 Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Tested applications: FC, ICC, IF, IHC
• Reactivity: Human
• Immunogen: Between 1200 and 1250
• Concentration: 50 µg/ml
• Dilution range: IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections.; IHC-IF - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC-IF - 1:100 - 1:500
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: Ki-67
• UniProt ID: P46013
• NCBI: NP_002408.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: PPP1R105; antigen identified by monoclonal antibod
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Ki-67 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Ki-67 (shaded) in MOLT4 cells by flow cytometry. Antibody: Rabbit anti-Ki-67 recombinant monoclonal (orb1519949) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human Ki-67 by immunocytochemistry. Sample: FFPE section of MOLT-4 cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of human Ki-67 by immunocytochemistry. Sample: acetone/methanol fixed Hela cells. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949) used at 1:100. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG. Counterstain: DAPI.

Detection of human Ki-67 (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-Ki-67 recombinant monoclonal antibody (orb1519949) used at 1:500. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).