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Rabbit anti-IP3R1 Antibody
Catalog Number: orb1527112
| Catalog Number | orb1527112 |
|---|---|
| Category | Antibodies |
| Description | IP3R1 Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | IHC, IP, WB |
| Reactivity | Human, Mouse |
| Immunogen | Between 2708 and C-terminus |
| Antibody Type | Primary Antibody |
| Concentration | 200 µg/ml |
| Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.; IHC-IF - 1:50 - 1:250 |
| Form/Appearance | Whole IgG |
| Conjugation | Unconjugated |
| Target | IP3R1 |
| UniProt ID | Q14643 |
| NCBI | Q14643.2 |
| Storage | 2 - 8°C |
| Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
| Alternative names | IP3R1; inositol 1,4,5-trisphosphate receptor type Read more... |
| Note | For research use only |
| Application notes | Format: Whole IgG |
| Expiration Date | 12 months from date of receipt. |
Detection of human IP3R1 by western blot. Samples: Whole cell lysate (5, 15, and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-IP3R1 antibody orb1527112 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human IP3R1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-IP3R1 antibody orb1527112 used for IP at 3 µg/mg lysate.
Detection of mouse IP3R1 by immunohistochemistry.Sample: FFPE section of mouse cerebellum. Antibody: Affinity purified rabbit anti-IP3R1 (Cat. No. orb1527112) used at a dilution of 1: 100 (2µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100. Counterstain: DAPI (blue).
Detection of mouse IP3R1 by immunohistochemistry.Sample: FFPE section of mouse cerebellum. Antibody: Affinity purified rabbit anti-IP3R1 (Cat. No. orb1527112) used at a dilution of 1: 200 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).
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