Rabbit anti-IP3R1 Antibody

SKU: orb1527111

Description

IP3R1 Antibody

Images & Validation

• Tested Applications: IHC, IP, WB
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 5 µg/mg lysate; IHC - 1:100 - 1:500. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.; IHC-IF - 1:50 - 1:250
• Reactivity: Human, Mouse
• Application Notes:
  Format: Whole IgG

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: Between 2708 and C-terminus
• Target: IP3R1

Storage & Handling

• Storage: 2 - 8°C
• Form/Appearance: Whole IgG
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Concentration: 200 µg/ml
• Disclaimer: For research use only

Alternative Names

IP3R1; inositol 1,4,5-trisphosphate receptor type 1; type 1 InsP3 receptor; type 1 inositol 1,4,5-trisphosphate receptor; SCA29; SCA16; SCA15; protein phosphatase 1, regulatory subunit 94; PPP1R94; IP3R; IP3 receptor isoform 1; ACV; INSP3R1; inositol 1,4,5-triphosphate receptor, type 1; CLA4; IP3 receptor; IP3R 1

Detection of human IP3R1 by western blot. Samples: Whole cell lysate (5, 15, and 50 µg) from HeLa cells. Antibody: Affinity purified rabbit anti-IP3R1 antibody orb1527111 used at 0.04 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Detection of human IP3R1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-IP3R1 antibody orb1527111 used for IP at 3 µg/mg lysate.

Detection of mouse IP3R1 by immunohistochemistry.Sample: FFPE section of mouse cerebellum. Antibody: Affinity purified rabbit anti-IP3R1 (Cat. No. orb1527111) used at a dilution of 1: 200 (1µg/ml). Detection: DAB. Counterstain: IHC Hematoxylin (blue).

Detection of mouse IP3R1 by immunohistochemistry.Sample: FFPE section of mouse cerebellum. Antibody: Affinity purified rabbit anti-IP3R1 (Cat. No. orb1527111) used at a dilution of 1: 100 (2µg/ml). Detection: Red-fluorescent goat anti-rabbit IgG-heavy and light chain cross-adsorbed Antibody DyLight® 594 Conjugated used at a dilution of 1: 100. Counterstain: DAPI (blue).

Quick Database Links

Gene Symbol

IP3R1

UniProt

Q14643

RefSeq:Q14643.2