Rabbit anti-HSF1 Antibody, Affinity Purified

• Catalog Number: orb1525317
• Category: Antibodies
• Description: HSF1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human
• Immunogen: Between 479 and C-terminus
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: HSF1
• UniProt ID: Q00613
• NCBI: NP_005517.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: HSTF1; heat shock factor protein 1; Heat shock tra
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human HSF1 by western blot and immunoprecipitation. Samples: Whole cell lysate from HeLa (15 and 50 µg for WB; 1 mg for IP, 20% of IP loaded) , Jurkat (J; 50 µg) and HEK293T (T; 50 µg) cells. Antibodies: Affinity purified rabbit anti-HSF1 antibody orb1525317 used for WB at 0.04 µg/ml (A) and 1 µg/ml (B) and used for IP at 6 µg/mg lysate. HSF1 was also immunoprecipitated by rabbit anti-HSF1 antibodies, which recognize upstream epitopes. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-HSF1 Cat. No. orb1525322 used at a dilution of 1: 5, 000 (0.2µg/ml) (left) , Cat. No. orb1525320 used at a dilution of 1: 5, 000 (0.2µg/ml) (center) and Cat. No. orb1525317 used at a dilution of 1: 1, 000 (0.2µg/ml) (right). Detection: DAB

Detection of human HSF1 by immunohistochemistry. Samples: FFPE sections of human breast carcinoma. Antibody: Affinity purified rabbit anti-HSF1 Cat. No. orb1525322 used at a dilution of 1: 1, 000 (1µg/ml) (left) , Cat. No. orb1525320 used at a dilution of 1: 1, 000 (1µg/ml) (center) and Cat. No. orb1525317 used at a dilution of 1: 200 (1µg/ml) (right). Detection: DAB