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Catalog Number | orb699957 |
---|---|
Category | Antibodies |
Description | Rabbit polyclonal antibody against Rabbit anti-Goat IgG (H&L) conjugated to AP |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | ELISA, ICC, IHC, WB |
Reactivity | Goat |
Immunogen | Purified Goat IgG, whole molecule |
Dilution range | 1 : 500-1 :2000 (ELISA), 1 : 50-1 : 5000 (ICC), 1 : 20 -1 : 2000 (IHC), 1 : 500-1 :2000 (WB) |
Form/Appearance | Liquid |
Purity | Immunogen affinity purified rabbit IgG. |
Conjugation | ALP |
Storage | Non-diluted antibody is stable for 4 years at 2-8°C. For storage at -20°C dilute antibody solution with an equal volume of glycerol to obtain final glycerol concentration of 50 % to prevent loss of enzymatic activity. Such solution will not freeze in -20°C. If you are using a 1:5000 dilution prior to diluting with glycerol, then you would need to use a 1:2500 dilution after adding glycerol. Prepare working dilution prior to use and then discard, Be sure to mix well but without foaming. |
Note | For research use only |
Application notes | No reactivity is observed to non-immunoglobulin goat serum proteins based in immunoelectrophoresis. BSA and milk have to be replaced by other blocking reagents, like doneky serum or commercial formulations which are free from bovine IgG. |
Expiration Date | 12 months from date of receipt. |
Donkey | |
Polyclonal | |
ALP |
ELISA, ICC, IHC, WB | |
Goat | |
Rabbit | |
Polyclonal | |
ALP |