Rabbit anti-EGFR Recombinant Monoclonal Antibody

• Catalog Number: orb1784586
• Category: Antibodies
• Description: Rabbit anti-EGFR Recombinant Monoclonal Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR252L
• Tested applications: FC, ICC, IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 1160 and C-terminus
• Concentration: 1000 µg/ml
• Dilution range: WB: 1:1000, IP: 6 µl/mg lysate, IHC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections, ICC: 1:100 to 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE cell sections
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: EGFR
• UniProt ID: P00533
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.09% Sodium Azide, rAlbumin-Free
• Alternative names: erb-b2 receptor tyrosine kinase 1; proto-oncogene
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human EGFR by western blot. Samples: Whole cell lysate (10 µg) from GaMG, HeLa, OVCAR-8, MCF-7, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of mouse EGFR by western blot. Samples: Whole cell lysate (10 µg) from AML12, NIH 3T3, and mIMCD-3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Detection of human EGFR by immunohistochemistry. Sample: FFPE section of human placenta. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586). Secondary: HRP-conjugated goat anti-rabbit IgG.

Detection of mouse EGFR (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human EGFR (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Detection of human EGFR by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg per IP; 10% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586) used for IP at 6 µl per reaction.

Detection of human EGFR by immunocytochemistry. Sample: FFPE section of A-431 cells. Antibody: Rabbit anti-EGFR recombinant monoclonal antibody (orb1784586). Secondary: HRP-conjugated goat anti-rabbit IgG.