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Catalog Number | orb1530063 |
---|---|
Category | Antibodies |
Description | DDX1 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human |
Immunogen | Between 690 and C-terminus |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | DDX1 |
UniProt ID | Q92499 |
NCBI | AAH12739.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | DEAD box protein retinoblastoma; ATP-dependent RNA Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human and mouse DDX1 by western blot.Samples: Whole cell lysate from HeLa, HEK293T, Jurkat, TCMK-1, and NIH 3T3 cells. Antibody: Affinity purified rabbit anti-DDX1 antibody orb1530063 at 0.04 µg/ml.
Detection of human DDX1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-DDX1 used at a dilution of 1:200 (1µg/ml).
Detection of mouse DDX1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-DDX1 used at a dilution of 1:200 (1µg/ml).
Detection of human DDX1 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-DDX1 antibody.
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |