Rabbit anti-CD8 alpha Recombinant Monoclonal Antibody

• Catalog Number: orb1519887
• Category: Antibodies
• Description: CD8 alpha Antibody
• Species/Host: Rabbit
• Clonality: Recombinant
• Clone Number: BLR044F
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human
• Immunogen: Between 185 and C-terminus
• Antibody Type: Primary Antibody
• Concentration: 250 µg/ml
• Dilution range: Flow-Cyt Fixed in 4% formaldehyde and permeabilized with 90% methanol. 0.5 µl per 1 x 10^6 cells. Immunohistochemistry (IHC) 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections. IHC-IF 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. Multiplex Immunofluorescence (mIF) 1:250. Western Blot (WB) 1:1000
• Form/Appearance: Whole IgG
• Purity: Recombinant antibody was purified from cell culture supernatant
• Conjugation: Unconjugated
• Target: CD8 alpha
• UniProt ID: P01732
• NCBI: NP_001759.3
• Storage: 2 - 8°C
• Buffer/Preservatives: Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: Leu2 T-lymphocyte antigen; T-lymphocyte differenti
• Background: The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human CD8 alpha by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG). Substrate: DAB

Detection of human CD8 alpha by immunhistochemistry. Sample: FFPE section of human melanoma. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:100. Secondary: DyLight® 594-conjugated goat anti-rabbit IgG

Detection of human CD8 alpha (red) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: Opal™. Counterstain: DAPI (blue).

Detection of human CD8 alpha by western blot. Samples: Whole cell lysate (50 µg) from CD4+ T-cells, CD8+ T-cells, Jurkat, MOLT-4, RPMI-8226, MJ, C5/MJ, KG-1, SR, and K-562 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD8 alpha recombinant monoclonal antibody used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Recombinant monoclonal antibodies to CD3e and CD4