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Catalog Number | orb1519905 |
---|---|
Category | Antibodies |
Description | CD44 Antibody |
Species/Host | Rabbit |
Clonality | Recombinant |
Tested applications | FC, ICC, IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 692 and C-terminus |
Concentration | 50 µg/ml |
Dilution range | WB - 1:1000; IP - 20 ul/1mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | CD44 |
UniProt ID | P16070 |
NCBI | NP_000601.3 |
Storage | 2 - 8°C |
Buffer/Preservatives | Borate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | MDU2; hyaluronate receptor; IN; Indian blood group Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human CD44 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, 786-O, HEK293T, A-549, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody.
Detection of mouse CD44 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.
Detection of human CD44 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
Detection of mouse CD44 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human CD44 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.
Detection of human CD44 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used for IP at 20 µl/mg lysate.
Detection of human CD44 in FFPE 786-O cells by ICC. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.
FC, ICC, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |
FC, ICC, IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Recombinant | |
Unconjugated |