Cart summary

You have no items in your shopping cart.

Rabbit anti-CD44 Recombinant Monoclonal Antibody [BLR038F]

Catalog Number: orb1519905

DispatchUsually dispatched within 5-10 working days
$ 670.00
Catalog Numberorb1519905
CategoryAntibodies
DescriptionCD44 Antibody
Species/HostRabbit
ClonalityRecombinant
Tested applicationsFC, ICC, IHC, IP, WB
ReactivityHuman, Mouse
ImmunogenBetween 692 and C-terminus
Concentration50 µg/ml
Dilution rangeWB - 1:1000; IP - 20 ul/1mg lysate; IHC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.; ICC - 1:100 to 1:500. Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE cell sections.
Form/AppearanceWhole IgG
ConjugationUnconjugated
TargetCD44
UniProt IDP16070
NCBINP_000601.3
Storage2 - 8°C
Buffer/PreservativesBorate Buffered Saline (BBS) pH 8.2 with 0.1% rAlbumin and 0.09% Sodium Azide
Alternative namesMDU2; hyaluronate receptor; IN; Indian blood group
Read more...
NoteFor research use only
Application notesFormat: Whole IgG
Expiration Date12 months from date of receipt.
Rabbit anti-CD44 Recombinant Monoclonal Antibody [BLR038F]

Detection of human CD44 by western blot. Samples: Whole cell lysate (10 µg) from HeLa, 786-O, HEK293T, A-549, and KG-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-COPB2 antibody.

Rabbit anti-CD44 Recombinant Monoclonal Antibody [BLR038F]

Detection of mouse CD44 by western blot. Samples: Whole cell lysate (10 µg) from NIH 3T3, CT26, CH27, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody.

Rabbit anti-CD44 Recombinant Monoclonal Antibody [BLR038F]

Detection of human CD44 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.

Rabbit anti-CD44 Recombinant Monoclonal Antibody [BLR038F]

Detection of mouse CD44 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Rabbit anti-CD44 Recombinant Monoclonal Antibody [BLR038F]

Detection of human CD44 (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) or isotype control (unshaded). Secondary: DyLight® 650-conjugated goat anti-rabbit IgG.

Rabbit anti-CD44 Recombinant Monoclonal Antibody [BLR038F]

Detection of human CD44 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905) used for IP at 20 µl/mg lysate.

Rabbit anti-CD44 Recombinant Monoclonal Antibody [BLR038F]

Detection of human CD44 in FFPE 786-O cells by ICC. Antibody: Rabbit anti-CD44 recombinant monoclonal antibody (orb1519905). Secondary: HRP-conjugated goat anti-rabbit IgG. Substrate: DAB.